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short interference rna sihoxb-as3  (Shanghai GenePharma)
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Shanghai GenePharma short interference rna sihoxb-as3
Short Interference Rna Sihoxb As3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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lentivirus expressing rna interference targeting hoxa-as3  (Shanghai GenePharma)
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Shanghai GenePharma lentivirus expressing rna interference targeting hoxa-as3
Lentivirus Expressing Rna Interference Targeting Hoxa As3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirna si-hoxc-as3-1  (Shanghai GenePharma)
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Shanghai GenePharma sirna si-hoxc-as3-1
Sirna Si Hoxc As3 1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirnas against magi2-as3  (Shanghai GenePharma)
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Shanghai GenePharma sirnas against magi2-as3
Sirnas Against Magi2 As3, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sirnas si-hoxb-as3#2  (Shanghai GenePharma)
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Shanghai GenePharma sirnas si-hoxb-as3#2
Sirnas Si Hoxb As3#2, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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si-hoxb-as3#1  (Shanghai GenePharma)
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Shanghai GenePharma si-hoxb-as3#1
Si Hoxb As3#1, supplied by Shanghai GenePharma, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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si-magi2-as3  (Ribobio co)
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Ribobio co si-magi2-as3
Si Magi2 As3, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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fluorescent probes of hoxc-as3 for rna fish  (Ribobio co)
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Ribobio co fluorescent probes of hoxc-as3 for rna fish
Fluorescent Probes Of Hoxc As3 For Rna Fish, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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chi-square test  (CH Instruments)
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CH Instruments chi-square test
Chi Square Test, supplied by CH Instruments, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoxb-as3 shrnas  (Broad Institute Inc)
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Broad Institute Inc hoxb-as3 shrnas
Hoxb As3 Shrnas, supplied by Broad Institute Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hoxa3  (Santa Cruz Biotechnology)
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Santa Cruz Biotechnology hoxa3
a qPCR analyses of the mRNA expression of <t>HOXA3</t> following HOXA-AS3 suppression for 48 h. b , c Western blotting detection of the protein levels of HOXA3 following HOXA-AS3 suppression for 48 h. d RNA pull-down assay and western blotting detection of HOXA-AS3 binding to the HOXA3 protein. (L lysate load, FT flow-through; E eluate). e Mass spectrum detection of HOXA-AS3 binding to the HOXA3 protein. f RNA pull-down assay and qPCR detection of HOXA-AS3 binding to the mRNA of HOXA3. (L lysate load, FT flow-through, E eluate). g RNA immunoprecipitation detection of the enrichment of HOXA-AS3 using an anti-HOXA3 antibody. (* p < 0.05, ** p < 0.01, *** p < 0.001)
Hoxa3, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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transfection vectors pcdna3.1-magi2-as3  (Ribobio co)
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Ribobio co transfection vectors pcdna3.1-magi2-as3
a qPCR analyses of the mRNA expression of <t>HOXA3</t> following HOXA-AS3 suppression for 48 h. b , c Western blotting detection of the protein levels of HOXA3 following HOXA-AS3 suppression for 48 h. d RNA pull-down assay and western blotting detection of HOXA-AS3 binding to the HOXA3 protein. (L lysate load, FT flow-through; E eluate). e Mass spectrum detection of HOXA-AS3 binding to the HOXA3 protein. f RNA pull-down assay and qPCR detection of HOXA-AS3 binding to the mRNA of HOXA3. (L lysate load, FT flow-through, E eluate). g RNA immunoprecipitation detection of the enrichment of HOXA-AS3 using an anti-HOXA3 antibody. (* p < 0.05, ** p < 0.01, *** p < 0.001)
Transfection Vectors Pcdna3.1 Magi2 As3, supplied by Ribobio co, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


a qPCR analyses of the mRNA expression of HOXA3 following HOXA-AS3 suppression for 48 h. b , c Western blotting detection of the protein levels of HOXA3 following HOXA-AS3 suppression for 48 h. d RNA pull-down assay and western blotting detection of HOXA-AS3 binding to the HOXA3 protein. (L lysate load, FT flow-through; E eluate). e Mass spectrum detection of HOXA-AS3 binding to the HOXA3 protein. f RNA pull-down assay and qPCR detection of HOXA-AS3 binding to the mRNA of HOXA3. (L lysate load, FT flow-through, E eluate). g RNA immunoprecipitation detection of the enrichment of HOXA-AS3 using an anti-HOXA3 antibody. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Journal: Oncogenesis

Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells

doi: 10.1038/s41389-019-0170-y

Figure Lengend Snippet: a qPCR analyses of the mRNA expression of HOXA3 following HOXA-AS3 suppression for 48 h. b , c Western blotting detection of the protein levels of HOXA3 following HOXA-AS3 suppression for 48 h. d RNA pull-down assay and western blotting detection of HOXA-AS3 binding to the HOXA3 protein. (L lysate load, FT flow-through; E eluate). e Mass spectrum detection of HOXA-AS3 binding to the HOXA3 protein. f RNA pull-down assay and qPCR detection of HOXA-AS3 binding to the mRNA of HOXA3. (L lysate load, FT flow-through, E eluate). g RNA immunoprecipitation detection of the enrichment of HOXA-AS3 using an anti-HOXA3 antibody. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: Small interfering RNAs (siRNAs) targeting HOXA-AS3 (si-HOXA-AS3-651, si-HOXA-AS3-728, si-HOXA-AS3-3507), HOXA3 (#sc-38675, Santa Cruz Biotechnology) or negative control (NC) siRNAs were transfected into NSCLC cells using Lipofectamine 3000.

Techniques: Expressing, Western Blot, Pull Down Assay, Binding Assay, RNA Immunoprecipitation

a – d CCK-8 assay of the viability of NSCLC cell lines following cisplatin treatment (0, 1.049, 2.097, 4.195, 8.389, 16.779 μM) under HOXA3 suppression for 48 h. e – f EDU assay of the proliferation of NSCLC cell lines under the IC 50 of cisplatin under HOXA3 suppression for 48 h. All scale bars indicate 50 μm (Green, EDU staining of dividing cells; blue, Hoechest 33342 staining of nuclei). g , h Flow cytometry detection of the apoptosis rate of NSCLC cell lines under the IC 50 of cisplatin under HOXA3 suppression for 48 h. i – m Western blotting of HOXA3, E-cadherin, and Vimentin levels following HOXA3 suppression for 48 h. n Western blotting of Twist1 levels following HOXA3 suppression for 48 h. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Journal: Oncogenesis

Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells

doi: 10.1038/s41389-019-0170-y

Figure Lengend Snippet: a – d CCK-8 assay of the viability of NSCLC cell lines following cisplatin treatment (0, 1.049, 2.097, 4.195, 8.389, 16.779 μM) under HOXA3 suppression for 48 h. e – f EDU assay of the proliferation of NSCLC cell lines under the IC 50 of cisplatin under HOXA3 suppression for 48 h. All scale bars indicate 50 μm (Green, EDU staining of dividing cells; blue, Hoechest 33342 staining of nuclei). g , h Flow cytometry detection of the apoptosis rate of NSCLC cell lines under the IC 50 of cisplatin under HOXA3 suppression for 48 h. i – m Western blotting of HOXA3, E-cadherin, and Vimentin levels following HOXA3 suppression for 48 h. n Western blotting of Twist1 levels following HOXA3 suppression for 48 h. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: Small interfering RNAs (siRNAs) targeting HOXA-AS3 (si-HOXA-AS3-651, si-HOXA-AS3-728, si-HOXA-AS3-3507), HOXA3 (#sc-38675, Santa Cruz Biotechnology) or negative control (NC) siRNAs were transfected into NSCLC cells using Lipofectamine 3000.

Techniques: CCK-8 Assay, EdU Assay, Staining, Flow Cytometry, Western Blot

a – d CCK-8 assay of the viability of NSCLC cell lines following cisplatin treatment (0, 1.049, 2.097, 4.195, 8.389, 16.779 μM) under HOXA-AS3 + HOXA3, or HOXA-AS3 only suppression for 48 h. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Journal: Oncogenesis

Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells

doi: 10.1038/s41389-019-0170-y

Figure Lengend Snippet: a – d CCK-8 assay of the viability of NSCLC cell lines following cisplatin treatment (0, 1.049, 2.097, 4.195, 8.389, 16.779 μM) under HOXA-AS3 + HOXA3, or HOXA-AS3 only suppression for 48 h. (* p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: Small interfering RNAs (siRNAs) targeting HOXA-AS3 (si-HOXA-AS3-651, si-HOXA-AS3-728, si-HOXA-AS3-3507), HOXA3 (#sc-38675, Santa Cruz Biotechnology) or negative control (NC) siRNAs were transfected into NSCLC cells using Lipofectamine 3000.

Techniques: CCK-8 Assay

a Growth curve of tumor volumes and b Growth curve of body weight calculated every 2 days after inoculation. c Tumor size after the tumors was harvested. d Tumor weight after the tumors was harvested. e – f Representative images of immunohistochemical detection of the proliferation index Ki-67 in vivo. g , h Representative images of immunohistochemical detection of the apoptosis index TUNEL in vivo. i , j Western blotting detection of HOXA3 in vivo. k qPCR detection of the mRNA expression levels of HOXA3 and HOXA-AS3 in vivo. (* vs cisplatin, * p < 0.05, ** p < 0.01, *** p < 0.001)

Journal: Oncogenesis

Article Title: LncRNA HOXA-AS3 confers cisplatin resistance by interacting with HOXA3 in non-small-cell lung carcinoma cells

doi: 10.1038/s41389-019-0170-y

Figure Lengend Snippet: a Growth curve of tumor volumes and b Growth curve of body weight calculated every 2 days after inoculation. c Tumor size after the tumors was harvested. d Tumor weight after the tumors was harvested. e – f Representative images of immunohistochemical detection of the proliferation index Ki-67 in vivo. g , h Representative images of immunohistochemical detection of the apoptosis index TUNEL in vivo. i , j Western blotting detection of HOXA3 in vivo. k qPCR detection of the mRNA expression levels of HOXA3 and HOXA-AS3 in vivo. (* vs cisplatin, * p < 0.05, ** p < 0.01, *** p < 0.001)

Article Snippet: Small interfering RNAs (siRNAs) targeting HOXA-AS3 (si-HOXA-AS3-651, si-HOXA-AS3-728, si-HOXA-AS3-3507), HOXA3 (#sc-38675, Santa Cruz Biotechnology) or negative control (NC) siRNAs were transfected into NSCLC cells using Lipofectamine 3000.

Techniques: Immunohistochemical staining, In Vivo, TUNEL Assay, Western Blot, Expressing